MON 87411 enriched mRNA was reverse transcribed using the
Omniscript Reverse Transcription kit (Qiagen, Valencia, CA). An
anchored oligo dT primer that is a random nucleotide sequence that
causes the oligo dT primer to anneal to the start of the poly (A) tail
rather than a position further downstream was used to produce
cDNA. The manufacturer's specifications were followed with the
exception of the incubation time (reaction was run for 30 min
instead of 60 min).