For agar cultures, besides some shelves and climatization, no other provisions arerequired. Liquid cultures, however, if submersed, require sufficient continuous aeration.Using Erlenmeyer flasks as culture vessels, rotary shakers with about 100 rpmusually give good results (Fig. 3.1 ). An interesting setup for liquid cultures is adevice called an auxophyton, developed in the early 1950s by the Steward group atCornell University (Fig. 3.2 ). Here, wooden discs with clips are mounted onto