In pyrosequencing (which employs in

In pyrosequencing (which employs instrumentation made by 454 Life Sciences, Inc. and also called 454 sequencing), segments of the DNA are immobilized on the surfaces of microscopic plastic beads (one DNA molecule per bead) that are deposited in small wells in a fiber-optic slide with one bead per well. A primer and DNA polymerase are added, and then a dNTP is introduced. If DNA polymerase adds that nucleotide to the growing DNA strand, pyrophosphate is released and undergoes a chemical reaction involving the firefly enzyme luciferase, which generates a flash of light. A detector records whether light is produced in the presence of a particular dNTP. In this way, the sequence of nucleotides complementary to the template strand can be deduced, and no electrophoretic separation is needed. Pyrosequencing can accurately determine reads of up to 700 nucleotides, somewhat shorter than the reads determined by chain-terminator sequencing. The fiber-optic slides contain numerous wells so that as many as ~1 million templates can be sequenced simultaneously. Consequently, the pyrosequencing system is ~1000-fold faster than the most advanced chain-terminator sequencing systems.

結果 (繁體中文) 2:

在熱測序（使用由454生命科學公司製造的儀器，也稱為454測序），DNA片段被固定在微觀塑膠珠（每個珠子一個DNA分子）的表面，這些微量沉積在小井中。光纖滑動，每井有一個珠子。加入引物和DNA聚合酶，然後引入dNTP。如果DNA聚合酶將核苷酸添加到生長的DNA鏈中，則釋放焦磷酸酯，並經歷涉及螢火蟲酶螢光素酶的化學反應，從而產生閃光。探測器記錄光是否在特定 dNTP 存在時產生。這樣，可以推匯出與範本鏈互補的核苷酸序列，無需電泳分離。熱測序可以準確地確定高達700個核苷酸的讀取，比鏈終端子測序確定的讀取略短。光纖滑軌包含大量井，因此可同時對多達 100 萬個範本進行排序。因此，熱測序系統比最先進的鏈端器測序系統快約 1000 倍。

結果 (繁體中文) 3:

在焦磷酸測序（使用454生命科學公司製造的儀器，也被稱為454測序）中，DNA片段被固定在顯微鏡下的塑膠珠（每珠一個DNA分子）的表面上，塑膠珠沉積在光纖玻片的小孔中，每孔一個DNA分子。加入引子和dna聚合酶，然後引入dntp。如果DNA聚合酶將該核苷酸添加到正在生長的DNA鏈中，焦磷酸鹽就會被釋放，並經歷一種涉及螢火蟲酶螢光素酶的化學反應，產生一道閃光。檢測器記錄在特定dntp存在下是否產生光。這樣就可以推匯出與範本鏈互補的核苷酸序列，而不需要電泳分離。焦磷酸測序法可以準確地測定多達700個核苷酸的讀值，比鏈式終止測序法測定的讀值略短。光纖載玻片含有許多孔，囙此可以同時對多達100萬個範本進行測序。囙此，焦磷酸測序系統比最先進的鏈終止測序系統快1000倍。