Protoplast cultures (see Chap. 5)In this approach, initially the cell wall of isolated cells is enzymatically removed,i.e., “naked” cells are obtained (Fig. 1.2 ), and the explant is transformed into asingle-cell culture. To prevent cell lysis, this has to be done under hypertonic conditions.This method has been used to study processes related to the regeneration ofthe cell wall, and to better understand its structure.