It is well-known that an increase o

It is well-known that an increase of intracellular ROS can lead to
apoptosis. On the other hand, a decrease of ROS can also ruin the
stability of mitochondria, which is followed by a loss of mitochondrial
membrane potential, release of cytochrome c into cytosol, and
cascade activation of caspases.27,28,30,32 The occurrence of the
apoptotic process should be confirmed by the presence of caspase-
3 and -9 (which played a crucial role and was a key element
in the execution phase of apoptosis). The enzyme activities of caspase-
3 and -9 were determined by spectrophotometry (Fig. 9).
After incubation with compound 7b for 24 h, the activities of caspase-
3 and -9 increased compared with the control. As shown in
Figure 9, the results clearly showed that an induction of cell apoptosis
took place when the cells were treated with compound 7b,
and the stimulation of caspase-3 and -9 activities both increased
in a time dependent manner from 3 to 24 h.

結果 (中文) 1:

它是知名的细胞内 ROS 增加可以导致细胞凋亡。另一方面，ROS 的减少也可以毁了随后的损失的线粒体的线粒体，稳定性膜电位、 细胞色素 c 的释放到细胞质内和级联的 caspases.27,28,30,32 活化的发生细胞凋亡过程应证实的半胱氨酸蛋白酶-存在3 和 9 (其中发挥关键的作用，是一个关键因素在执行阶段的细胞凋亡)。半胱氨酸蛋白酶的酶活性3 和 9 分光光度法测定 (图 9)。与复合 7b 24h，活动的半胱氨酸蛋白酶-孵育后3 和 9 增加与对照相比。如中所示图 9，结果清楚地表明，诱导细胞凋亡发生时，细胞治疗复合 7b，和半胱氨酸蛋白酶-3 和-9 活动刺激增加在时间依赖的方式从 3 到 24 小时。

結果 (中文) 3:

众所周知，细胞内活性氧的增加可导致细胞凋亡。另一方面，减少ROS也可以破坏线粒体的
稳定性，其次是损失的线粒体
膜电位，细胞色素c释放到细胞质中，与
Caspase级联反应的激活。27,28,30,32的
凋亡过程的发生应该由半胱氨酸天冬氨酸蛋白酶的存在证实
9和3（在细胞凋亡过程中起着关键作用，是细胞凋亡的关键因素）。酶活性的caspase - 3和- 9
采用分光光度法测定了（图9）。
与24 H化合物7b孵育后，活动
caspase - 3和- 9增加与控制。如图9所示，结果清楚地表明，诱导细胞凋亡发生时的细胞复合7B处理，
和caspase-3和刺激9活动增加
呈时间依赖性从3到24小时。