The fungi commonly used are listed in Table 508.6-I. These organisms were selected because of their ability to degrade materials, their worldwide distribution and their stability. These organisms have, where possible, been identified with respect to the materials to which they are known to attack. The European species can be found in Part Three (paragraph 3.5.11) of this standard, as well as in STANAG 4370, AECTP 308. a. Because the test item is not sterile before testing, other microorganisms will be present on the surfaces. When the test item is inoculated with the test fungi, both these and the other organisms will compete for available nutrients. It is not surprising to see organisms other than the test fungi growing on the test item at the end of the test. Hence, the need for trained personnel, e.g., mycologists, microbiologists, etc., to identify such situations. b. Add additional species of fungus to those required in this test method. However, if additional fungi are used, base their selection on prior knowledge of specific material deterioration. For example, Aureobasidium pullulans was once employed because of its known specificity for degrading paints. (It has since been deleted from some categories because of mutations to the strain.) c. Recommend only 5 or 6 species be used in each test. If additional species are needed, remove a species from the list. The more dominant species will prevail in testing, therefore adding more species will only increase test costs without an increase in deterioration to material. Discuss this issue with a microbiologist for the most effective approach. Test Facility. In addition to the standard requirements for test chambers, the following apply to chambers to be used for fungus tests. 4.1.1 Test chamber. Construct the chamber and accessories in such a manner as to prevent condensation from dripping on the test item. Filter-vent the chamber to the atmosphere to prevent the buildup of pressure and release of spores into the atmosphere.