However, BCS (P = 0.8), BCS change (P = 0.4), and DPP (P = 0.5) had no significant effects on Day 7 embryo size. A multivariate analysis indicated no effect of grade (1, 2, and 3) on the gene expression profile (P = 0.1). A multivariate analysis indicated no significant effects of Day 0 progesterone (P = 0.2), Day 7 progesterone (P = 0.5), BCS (P = 0.6), and DPP (P = 0.6) on the gene expression data (81 genes with gene expression greater than the limit of detection of the assay).