Microplastics were created from ten post-consumer items (Table SI.3). Fragments were produced with an electrical coffee bean grinder and dry-sieved through 63 and 600 μm stainless steel sieves. Particles <600 μm were retained. Fibres were obtained by plugging and cutting. Film/sheet was also cut. Thirty particles of each material were added to M. gigas tissue. Containers used for storing those particles prior to spiking were subsequently inspected for potentially left behind items. Spiked samples were re-frozen and treated like efficacy experiment specimens. Since small pore size for filtration is paramount when assessing human exposure rates, only the performance of the KOH digestion (10%, 60 °C) was tested (n = 4).