Effect of M. grandiflora L. flower extract on expression of melanogenesis-related proteins
To test whether M. grandiflora L. flower extract does regulate the expression of melanogenesis-related proteins,B16F10 melanoma cells were treated with α-MSH (100 nM) for 24 h, and then 10, 15, and 20% (v/v) of M. grandiflora L. flower extract or kojic acid (200 μM) was
added for another 24 h. MITF, tyrosinase, TRP-1, and TRP-2 levels were assayed by Western blot (Figure 3A).
It was found that M. grandiflora L. flower extract treatment led to a reduced level of tyrosinase and TRP-1 (Figure 3B), but the protein contents of MITF and TRP-2 were not obviously changed after treatment (Figure 3A).