Handling controls were prepared by

Handling controls were prepared by dosing ultrapure water (60 ml, n = 4) and kept at room temperature for 48 h. This was done to assess potential losses unrelated to the reagent, temperature or interaction with biological tissue. An additional water blank was run after filtering the samples to assess if any particles from previous samples could have contaminated subsequent ones. Samples were vacuum filtered (25-μm filters) and rinsed with 20 ml of ultrapure water. This amount was chosen because initial trials with tissue samples indicated that larger amounts of water were not filterable. During such trials, particles were observed stuck to the sample containers and filtration funnels. To enumerate potential losses, after sample filtration a new filter was placed in the unit and the sampling jars and funnel flushed with approximately 400 ml of ultrapure water.

結果 (繁體中文) 2:

處理控制裝置是通過給給超純水（60 ml，n = 4）製備的，並在室溫下保持48小時。這樣做是為了評估與試劑、溫度或與生物組織相互作用無關的潛在損失。過濾樣品後，又運行了一個水空白，以評估以前樣品中是否有任何顆粒可能污染了隨後的顆粒。樣品經過真空過濾（25μm篩檢程式），用20毫升超純水沖洗。之所以選擇這個量，是因為對組織樣本的初步試驗表明，大量的水是不可過濾的。在此類試驗中，觀察到顆粒粘在樣品容器和過濾漏斗上。為了列舉潛在的損失，在樣品過濾後，在裝置中放置了一個新的篩檢程式，取樣罐和漏斗用大約400毫升的超純水沖洗。

結果 (繁體中文) 3:

通過給藥超純水（60 ml，n=4）製備處理對照品，並在室溫下保持48 h。這樣做是為了評估與試劑、溫度或與生物組織的相互作用無關的潜在損失。在過濾樣品後，再進行一次水空白試驗，以評估先前樣品中的任何顆粒是否可能污染後續樣品。樣品經真空過濾（25μm篩檢程式）並用20 ml超純水沖洗。之所以選擇這個量，是因為對組織樣本的初步試驗表明，大量的水是不可過濾的。在這些試驗中，觀察到顆粒粘附在樣品容器和過濾漏斗上。為了計算潜在損失，在樣品過濾後，在裝置中放置一個新的篩檢程式，用大約400 ml超純水沖洗取樣罐和漏斗。