Chemotherapy•In 1942, the first chemotherapy reserach had revealedthat intravenous doses of mustard gas led to temporaryregression of a lymphoma.•In 1947 the utility of aminopterin (acted as anantagonist of folate metabolism) was treatedlymphoma patient and was the first documented cureof antimetabolite used to treat cancer.• Another class of antimetabolites affects normal cellfunction by interfering with microtubule assembly,either inhibiting or fostering it