a. Viability of spore suspension (1) Before preparing the composite spore suspension, inoculate sterile potato dextrose (or another nutrient agar) plates with 0.2 to 0.3 ml of the spore suspension of each of the individual fungus species. Use separate agar plates for each species. (2) Distribute the inoculum over the entire surface of the plate. (3) Incubate the inoculated potato dextrose agar plate at 30 ±1°C (86 + 2°F) for 7 to 10 days. (4) After the incubation period, check the fungus growth.