AO/EB staining. AO, which is a vital dye, can stain nuclear
DNA across an intact cell membrane, while EB can only stains cells
that had lost their membrane integrity. Therefore, after synchronous
treating with AO and EB, live cells will be evenly stained as
green (in the web version) and early apoptotic cells will be thickly
stained as green yellow or showgreen yellow fragments (in the web
version), while late apoptotic cells will be densely stained as orange
or display orange fragments and necrotic cells will be stained as
orange with no condensed chromatin.