Real-time qPCR reactions, using 10 ng of cDNA per reaction, two experiments, four replicates per experiment, were carried out on an ABI Instruments 7900HT qPCR detection system (Applied Biosystems, Foster City, CA, USA) using Platinum SYBR Green qPCR SuperMix-UDG with ROX (Life Technologies, Carlsbad, CA, USA).