However, it is important to note that in the current study, transference data were calculated 1 d after the last AF challenge was given, whereas previous research calculated transference data on the same day that an AF challenge was given. Also, AFM1 transfer values were similar to those from Maki et al. (2016) when an adsorbent was added to the diet. No difference was observed for AFM1 excretion when adsorbent was added to the diet. Increased milk yield, FCM, and ECM when adsorbent was added to the diet could explain the result that AFM1 excretion did not decrease during the AF challenge.