Briefly, mice were deeply anesthetized using a ketamine/xylazine/acepromazine mix and intracardially perfused with 4 °C slicing solution containing (in mM) 93 NMDG, 93 HCl, 30 NaHCO3, 25 glucose, 20 HEPES, 10 MgSO4, 5 sodium ascorbate, 2.5 KCl, 3 sodium pyruvate, 2 thiourea, 1.2 NaH2PO4 and 0.5 CaCl2 (pH 7.3–7.4, continuously oxygenated with 95% O2/5% CO2).