Ethyl acetate was prepared for MIC

Ethyl acetate was prepared for MIC as mentioned above. Brothmicro-dilution method was used to determine MIC. MHB mediumwas added into sterile 96 well plates (185 l for each well). The fol-lowing concentrations of the test extract, 2000, 1000, 500, 250, 125,62.5 and 31.25 g/ml, were added into 96 well plates. From eachinoculum culture suspension, 5 l was added into the wells. Strep-tomycin and DMSO were added as positive and negative controlsrespectively. Plates of 96 wells was incubated at 37◦C for 17 h.

結果 (繁體中文) 1:

乙酸乙酯為MIC製備如上所述。使用Brothmicro稀釋法來確定MIC。MHB mediumwas加入到無菌的96孔板（185？升每孔）。測試的提取物，2000，1000，500，250，和125,62.5 31.25微克/毫升的FOL-降脂濃度，加入到96孔板中。從eachinoculum文化懸架，5？l加入到井中。鏈球菌-tomycin和DMSO中加入作為陽性和陰性controlsrespectively。的96孔平板在37◦C溫育17小時。

結果 (繁體中文) 2:

如上文所述，為MIC製備了乙酸乙酯。採用微稀釋法測定MIC。MHB介質被添加到無菌的96孔板（每口井185升）。測試提取物的低濃度，2000，1000，500，250，125，62.5和31.25g/ml，被添加到96個孔板中。從每口井的懸浮液中，5升被添加到井中。鏈球菌和DMSO分別作為正對照和負對照添加。96口井的板在37°C孵育了17小時。

結果 (繁體中文) 3:

如上所述，為MIC製備乙酸乙酯。採用肉湯微量稀釋法測定MIC。將MHB培養基加入無菌96孔板中（每孔185l）。將下列濃度的試驗提取物2000、1000、500、250、125、62.5和31.25 g/ml加入96孔板中。從培養液中加入5L。鏈黴素和二甲基亞碸分別作為陽性對照和陰性對照。96個孔的培養板在37℃下培養17小時。