Greenhouse experiments using the system called Development and Assessment of Sustainable Humanosphere System located at the Uji Campus of Kyoto University were performed during March 2010–June 2010 and September 2010–March 2011. Four tobacco emitter plants (WT or NtOS2 plants) and four receiver lima bean plants were placed 30 or 60 cm apart in a chamber (200 cm wide, 300 cm long, 450 cm height) of the greenhouse, without climate control or air flow. During the 7-day treatments, the temperature ranged from 24 to 36uC during the day and from 16 to 24uC at night with an average day period from 5:30 AM to 6:30 PM. Each fumigation was independently replicated 4 times for a given set of experiments.