Atlantic mackerel was purchased fro

Atlantic mackerel was purchased from a local retailer (Wageningen, The Netherlands) and ground
in a meat grinder with a 4-mm die. Each batch of silage consisted of 100 g of ground mackerel to which 100 ml of an aqueous solution containing 2.4g l sucrose, 0.4 g l NaCl and 0.2 g l histamine dihydrochloride were added. The resulting mixture was thoroughly homogenised using a spatula and 2%(v/w) of an 18-h MRS broth (Oxoid, CM 359,Basingstoke, UK) culture of the designated LAB isolate was added. For the DAO experiment, 10 780 21 units kg DAO were added as well. Cultures 15.18 and 15.36 (both Lb. sakei) were used as an inoculum in the fish experiment, since they had been found to degrade histamine in the model system. Fish slurries
with added DAO were inoculated with a non-his-tamine degrading bacterium (Lb. curvatus 22.24). A control slurry was inoculated with isolate 22.24 only,to determine histamine autodegradation during ensil-

結果 (中文) 1:

从当地零售商 (瓦赫宁根，荷兰) 和地面购买大西洋鲭鱼在有 4 毫米模具绞肉机。每一批的青贮饲料包括地面鲭鱼 0.4 g l NaCl 和 0.2 g l 组胺二盐酸盐添加到含 2.4 g l 蔗糖水溶液的 100 毫升，100 克。产生的混合物彻底被同质化用抹刀加 2%(v/w) 指定的实验室分离 18 h 太太肉汤 (Oxoid 厘米 359 贝辛斯托克，英国) 文化。10 780 21 单位公斤街道也添加道试验。文化 15.18 和 15.36 (两磅 sakei) 被用作接种在鱼实验中，因为他们被发现在模型系统降低组胺。鱼浆与添加街道接种非他 tamine 有辱人格细菌 (磅 curvatus 22.24)。控制浆接种了隔离 22.24 只，以确定在 ensil-组胺 autodegradation

結果 (中文) 3:

大西洋鲭鱼是从当地零售商购买（瓦赫宁根，荷兰）和地面在一个4mm死绞肉机。每批青贮为100克，100毫升的地面鲭鱼含有2.4G L蔗糖水溶液，加入0.4克盐0.2克升二盐酸组胺是。由此产生的混合物用刮刀彻底化2%（V/W）的18小时的MRS肉汤（Oxoid，厘米359，贝辛斯托克，英国）加入隔离指定的实验室文化。为刀试验，780个10个21个单位公斤公斤道被添加为。培养15.18和15.36（两磅。sakei）作为鱼实验接种，因为他们已经找到了降低系统模型中的组胺。鱼浆补充道接种了非组胺降解菌（磅弯曲22.24）。控制浆接种分离出22.24只，在ensil确定组胺降解—