Although required, the nuclease act

Although required, the nuclease activities of Mre11 alone are not sufficient for resection. This requires the BRCA1 tumor suppressor and the CtIP protein, both bound to MRN. CtIP shares a limited region of homology with the Sae2 nuclease in Saccharomyces
cerevisiae, but unlike Sae, CtIP has not yet been shown to possess nuclease activities. Rather, CtIP interaction appears to be required to enhance Mre11 nuclease activity. For efficient resection in vivo, CtIP must interact with BRCA1 (refs. 19,20). It is the formation and dissolution of the MRN–CtIP–BRCA1 complex that provides the regulation of resection capacity (and thus homologous recombination capacity) throughout the cell cycle. This complex is only found in S and G2 phases

結果 (繁體中文) 2:

雖然要求，MRE11的核酸酶的活動本身並不足以切除。這就要求有BRCA1腫瘤抑制和CtIP蛋白，二者結合到MRN。CtIP共享同源性SAE2核酸酶在釀酒有限區域
酵母，但不同於SAE，CtIP尚未被證明具有核酸酶的活動。相反，CtIP相互作用似乎需要提高MRE11核酸酶活性。為了有效地切除體內，CtIP必須與BRCA1相互作用（參。19,20）。它是MRN-CtIP-BRCA1複雜，可提供的切除能力（從而同源重組的能力）在整個細胞週期調控的形成和溶解。這種複合物僅在S和G2期發現

結果 (繁體中文) 3:

雖然，MRE11核酸酶活性是不够的切除。這需要BRCA1腫瘤抑制和CtIP蛋白結合MRN。在釀酒酵母
CtIP股份與SAE2核酸同源性有限的區域，但與SAE、CtIP尚未被證明具有核酸酶活性。相反，CTIP互動似乎需要提高MRE11核酸酶活性。在體內有效的切除、CtIP必須與BRCA1（參。19、20）。這是和MRN–CtIP–BRCA1複雜提供切除容量調節溶解形成的（囙此同源重組能力）在整個細胞週期。這個複雜的是只發現在S和G2期