We next conducted experiments in a greenhouse. Hereafter, we focus on the lima bean system as a model case. As in the above open-flow system, uninfested lima bean plants were placed 30 cm apart from NtOS2 or WT plants (VOCos-receivers or VOCwt- receivers) in a greenhouse for 7 days without any climate control. We then evaluated oviposition of T. urticae eggs on the VOCos- receiver or VOCwt-receiver leaf sections and their capability of indirect defense by analyzing the emission levels of VOCs and the attraction of P. persimilis after T. urticae attack for 1 day.