Hoechst 33258 staining. Hoechst 332

Hoechst 33258 staining. Hoechst 33258, which stains the
cell nucleus, is a membrane permeable dye with blue fluorescence.
Live cells with uniformly light blue nuclei were obviously detected
under fluorescence microscope after treatment with Hoechst
33258, whereas apoptotic cells had bright blue nuclei due to karyopyknosis
and chromatin condensation. However, the nuclei of
dead cells could not be stained. A549 cells treated with compound
5f at 15 mMfrom 12 to 24 h were stained with Hoechst 33258. A549
cells not treated with the 5f was used as control at for 24 h. The
results were given in Fig. 2
As shown in Fig. 2, cells not treated with compound 5f were
normally blue (in the web version). It was worth noting that, for 5f
treatment, the cells displayed strong blue fluorescence and indicated
typical apoptotic morphology after 12 and 24 h. The observation
revealed that compounds 5f induced apoptosis against A549
cell lines, consistent with the results for AO/EB double staining.

結果 (中文) 1:

Hoechst 33258 染色。Hoechst 33258，污渍细胞细胞核，是蓝色荧光膜渗透性染料。显然检测活细胞与均匀轻蓝核赫司特治疗后荧光显微镜下33258，而细胞凋亡有明亮的蓝色细胞核，由于核固缩和染色质固缩。然而，原子核死细胞不被染色。A549 细胞治疗化合物5f 15 mMfrom 在 12 至 24 h hoechst 33258 染色。A549细胞不处理 5f 被用作 24 h.的控制在图 2 中给出了结果如图 2 所示，不治疗化合物 5f 细胞通常蓝色 (在 web 版本)。这是值得关注的，对于 5 楼治疗，细胞显示强蓝色荧光和表示典型的细胞凋亡形态后 12 和 24 h。观察透露，化合物 5f 诱导细胞凋亡对 A549细胞系，与 AO/EB 双染色的结果相一致。

結果 (中文) 3:

Hoechst 33258染色。Hoechst 33258染色，这
细胞核，是一种具有蓝色荧光染料活细胞膜渗透性。
均匀的淡蓝色的细胞核明显
Hoechst 33258荧光显微镜下
治疗后，而凋亡细胞有明亮的蓝色细胞核固缩，染色质浓缩，由于
。然而，核死亡细胞不能被染色。A549细胞复合
5F 15 mmfrom 12至24小时，用Hoechst 33258染色。A549细胞不
5F处理为对照，在24小时
结果图2
如图2所示，不与化合物5F处理的细胞
通常蓝（在网页版）。值得注意的是，
处理5F，细胞表现出较强的蓝色荧光，表明
典型的细胞凋亡形态，后12和24 h观察
表明化合物5F诱导的细胞凋亡与细胞系A549
，用AO/EB染色的结果一致。