Cell proliferation assay
Cell viability assay of B16F10 was performed using 3-(4,
5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide
(MTT) [31]. In brief, 1 × 104 cells/well was seeded into a
96-well plate. The cells were exposed to various concentrations
of M. grandiflora L. flower extract (final concentration
10, 12.5, 15, 17.5, 20%; v/v) for 24 h, the MTT
solution was added to the wells. The insoluble derivative
of MTT produced by intracellular dehydrogenase was
solubilized with ethanol-DMSO (1:1 mixture solution).
The absorbance of the wells at 570 nm was read using a
microplate reader