Cells were seeded at a concentratio

Cells were seeded at a concentration of 5  104 cell/mL in a
volume of 2 mL on a sterile cover slip in six-well tissue culture
plates. Following incubation, the medium was removed and
replaced with fresh medium plus 10% fetal bovine serum and
supplemented with compound 5f (15 mM). After the treatment
period, the cover slip with monolayer cells was inverted on a glass slide with 20 mL of AO/EB stain (100 mg/mL). Fluorescencewas read
on a Nikon ECLIPSETE2000-S fluorescence microscope (OLYMPUS
Co., Japan).

結果 (中文) 1:

细胞接种在 5 104 细胞/毫升浓度上六井组织培养中的无菌盖滑 2 毫升的量印版。后孵化，介质被删除和更换新鲜培养基加 10%胎牛血清和辅以化合物 5f (15 毫米)。治疗后期间，与单层细胞盖玻片倒置于 20 毫升的 AO/EB 染色 (100 毫克/毫升) 的玻璃幻灯片。Fluorescencewas 读尼康 ECLIPSETE2000-S 荧光显微镜 (奥林有限公司，日本)。

結果 (中文) 3:

细胞接种浓度为5  104细胞/毫升在
体积2 ml无菌盖玻片的六孔培养板
。孵育后，介质被
更换新鲜培养基加10%胎牛血清和
添加化合物5F（15毫米）。治疗结束后，与单层细胞的盖玻片倒置于与AO/EB染色玻片20毫升（100毫克/毫升）。读
是在尼康eclipsete2000-s荧光显微镜（Olympus
有限公司，日本）。