The objective of this study was to determine the effects of a commercially available aluminosilicate clay in a traditional lactation diet during an AF challenge (AFB1) on the presence of AFM1 in milk and urine, AFB1 in feces, production responses, blood chemistry, and liver inflammatory markers of multiparous lactating Holstein cows. The hypothesis was that cows receiving aluminosilicate clay would experience lower AF excretion in milk, urine, and feces and would therefore exhibit lower oxidative stress than cows not receiving adsorbent.