Figure 3 Effect of M. grandiflora L. flower extract on melanogenesis-related proteins expression.
(A) B16F10 cells were cultured with α-MSH (100 nM) for 24 h and then treated with various concentration of the flower extract (10, 15, 20%; v/v) or kojic acid (200 μM) for another 24
hr. Then the content of cellular MITF, tyrosinase, TRP-1 and TRP-2 proteins were analyzed by western blotting assay.
(B) The relative amounts of MITF, tyrosinase, TRP-1 and TRP-2 compared to total GAPDH were calculated and analyzed by Multi Gauge 3.0 software and the values
represented the mean of triplicate experiments ± S.D.