Aflatoxin M1 excretion in milk (μg/kg) was calculated in comparison with Maki et al. (2016) using the following equations: excretion (μg/kg) = concentration of AFM1 in milk on d 18 (μg) × milk yield on d 18 (kg), and AF transfer (%) = [AF excretion (μg/kg)/AFB1 challenge (μg/kg)] × 100.