Recently, oxidative damage to the m

Recently, oxidative damage to the mitochondrial membrane
due to increased generation of reactive oxygen species (ROS) has
been demonstrated to play a important role in apoptosis.28–30
Mitochondria has also been implicated as a source of ROS during
apoptosis. Reduced mitochondria membrane potential has recently
been shown to lead to increased generation of ROS and apoptosis.31
We herein studied the loss of mitochondrial transmembrane potential
resulting in the generation of ROS by assessing ROS generation
caused by target compound 7b in vitro, using the fluorescent
probe 2,7-dichlorofluorescein diacetate (DCF-DA) determined by
fluorescence microscopy. Treated with compound 7b, cells exhibited
stronger fluorescence intensity in cytoplasm, while not treated
with compound 7b, cells under the same experimental procedures
were used as control and the fluorescence detected in these cells
were weak and spread all over the cells. For fluorescence microscopy,
Figure 8 revealed that HeLa cells treated with the compound
7b appeared in stronger green fluorescence (in the web version),
indicating that compound 7b significantly induced apoptosis
against HeLa cell line. This phenomenon implied that the increment
of ROS might play a role as an early mediator in compound
7b induced apoptosis. These findings pointed to an effect of compound
7b on mitochondrial function and accumulation of ROS.
These features were cues for the induction of apoptosis.

結果 (中文) 1:

最近，氧化损伤线粒体膜由于增加生成的活性氧 (ros) 有已证明在 apoptosis.28–30 中发挥着重要的作用线粒体有也牵连的过程中活性氧的来源细胞凋亡。减少的线粒体膜电位最近被证明导致增加生成的活性氧和 apoptosis.31我们这里研究线粒体跨膜电位的损失在 ROS 的产生造成评估 ROS 的产生引起目标复合 7b 体外，使用荧光灯2，7-二氯荧光素二乙酸酯探针 (DCF DA) 测定荧光显微镜。用复合 7b 处理后，细胞展出较强的荧光强度在细胞质，在不治疗的同时与复合 7b，细胞进行相同的实验程序被用作控制和在这些细胞中检测到的荧光是弱和传得到处都细胞。荧光显微镜图 8 显示 HeLa 细胞治疗与化合物7b 出现在更强的绿色荧光，(在 web 版)指示该复合 7b 显著诱导细胞凋亡对 HeLa 细胞系。这种现象暗示增量ROS 的产生可能发挥作为一个早期的调解者在化合物中的作用7b 诱导细胞凋亡。这些研究结果指出，复方的影响对线粒体功能和积累的 ROS 7b。这些功能是诱导细胞凋亡的线索。

結果 (中文) 3:

最近，氧化损伤的线粒体膜，由于增加产生的活性氧（活性氧）的细胞凋亡中发挥了重要的作用。28，30的线粒体也被牵连在细胞凋亡的来源。减少线粒体膜电位最近已被证明会导致生成的活性氧和细胞凋亡的增加。31本文研究了线粒体跨膜电位
产生ROS的产生
评估体外目标化合物7b引起ROS的产生的损失，使用荧光探针
二氢二氯荧光素二乙酸酯（DCF-DA）的
荧光显微镜测定。复方7B处理，细胞胞浆内呈
更强的荧光强度，而不治疗
复方7B，相同的实验程序
下细胞作为对照，荧光检测这些细胞
弱，遍布细胞。荧光显微镜，
图8显示HeLa细胞与复合
7B处理强绿色荧光出现（在网页版），
表明化合物7b明显诱导细胞凋亡
对HeLa细胞。这种现象表明ROS的增加
可能作为复合
早期调解员7b诱导的细胞凋亡中发挥作用。这些研究结果指出，复合
7b对线粒体功能及活性氧的积累效应。
这些功能是诱导细胞凋亡的线索。