A blend of volatile organic compoun

A blend of volatile organic compounds (VOCs) emitted from plants induced by herbivory enables the priming of defensive
responses in neighboring plants. These effects may provide insights useful for pest control achieved with transgenic-plantemitted
volatiles. We therefore investigated, under both laboratory and greenhouse conditions, the priming of defense
responses in plants (lima bean and corn) by exposing them to transgenic-plant-volatiles (VOCos) including (E)-b-ocimene,
emitted from transgenic tobacco plants (NtOS2) that were constitutively overexpressing (E)-b-ocimene synthase. When lima
bean plants that had previously been placed downwind of NtOS2 in an open-flow tunnel were infested by spider mites,
they were more defensive to spider mites and more attractive to predatory mites, in comparison to the infested plants that
had been placed downwind of wild-type tobacco plants. This was similarly observed when the NtOS2-downwind maize
plants were infested with Mythimna separata larvae, resulting in reduced larval growth and greater attraction of parasitic
wasps (Cotesia kariyai). In a greenhouse experiment, we also found that lima bean plants (VOCos-receiver plants) placed
near NtOS2 were more attractive when damaged by spider mites, in comparison to the infested plants that had been placed
near the wild-type plants. More intriguingly, VOCs emitted from infested VOCos-receiver plants affected their conspecific
neighboring plants to prime indirect defenses in response to herbivory. Altogether, these data suggest that transgenicplant-
emitted volatiles can enhance the ability to prime indirect defenses via both plant-plant and plant-plant-plant
communications.

結果 (繁體中文) 2:

一個混合的揮發性有機化合物（VOC）的誘導取食植物發出的能防守的吸
鄰近植物的反應。這些影響可能啟發實現了與轉基因-plantemitted防蟲有用的
揮發物。因此，我們研究，實驗室和溫室條件下，防禦的塗刷
在植物中的反應（利馬豆和玉米）通過暴露於轉基因植物揮發物（VOCos），包括（E）-b羅勒烯，
從轉基因煙草植物發射（NtOS2），這些組成過表達（E）-b羅勒烯合酶。當利馬
豆植物，以前被放置順風NtOS2在一個開放式的流動通道被紅蜘蛛出沒，
他們更守紅蜘蛛和捕食蟎的吸引力，相較於出沒的植物
被放在順風野生型煙草植物。此類似地觀察到當NtOS2-順風玉米
植株侵染粘蟲幼蟲，從而降低幼蟲的生長和寄生的較大的吸引力
蜂（繭kariyai）。在溫室實驗中，我們還發現，利馬豆植物（VOCos-接收機植物）放置
在由葉蟎損壞，相比於侵染的植物已被放置在靠近NtOS2更具吸引力
接近野生型的植物。更有趣的是，揮發性有機化合物出沒VOCos接收器廠排放影響了他們的同種
相鄰的植物素間接防禦響應取食。總之，這些數據表明，transgenicplant-
發射揮發物可以增強通過既植物植物和植物植物植物的能力素間接防禦
通信。

結果 (繁體中文) 3:

一種揮發性有機化合物（VOCs）混合引起取食植物發出使周圍植物的防禦
響應啟動。這些影響可能提供有用的控制害蟲的轉基因plantemitted
揮發物取得的見解。囙此，我們調查，在實驗室和溫室條件下，吸的防禦反應在植物（利馬豆和玉米）暴露於轉基因植物揮發物（vocos）包括（E）- b-ocimene，
轉基因烟草植物排放（ntos2），組成過表達（E）- b-ocimene合成酶。當利馬
豆植物，以前一直放在順風的ntos2在開放式出沒蜘蛛，
他們更守蜘蛛和捕食蟎更具吸引力，在這
一直放在順風的出沒的野生型烟草植物的比較。這是同樣觀察到ntos2順風玉米
植物感染粘蟲幼蟲，從而降低幼蟲的生長和更大的吸引力
寄生黃蜂（蜂kariyai）。在溫室試驗中，我們還發現，利馬豆植物（vocos接收機植物）放
近ntos2更具吸引力，當被蜘蛛，在已經放
附近的野生型植物的侵染植物的比較。更有趣的是，揮發性有機化合物的排放從出沒的vocos接收機影響他們的同種植物
鄰近的植物素間接防禦草食動物。總之，這些資料表明，轉基因植物-
揮發物可以提高能力素間接防禦通過植物和植物的植物
通信。