Fraction 1 CIRJP inhibits extracell

Fraction 1 CIRJP inhibits extracellular Aβ1-40 and Aβ1-42 accumulation in N2a/APP695 cells.Several RJP fractions were separated from crude RJPs via the HPLC method. We chose the 1 CI fraction as theactive ingredient after preliminary functional verification. Our results showed that 1 CI RJP below 40 μg·ml-1 didnot affect N2a and N2a/APP695 cell viability during 48 hours (Fig. 3). Compared with N2a cells, N2a/APP695cells produced a large amount of extracellular Aβ1-40 and Aβ1-42. As shown in Fig. 4, N2a/APP695 cells culturedfor 48 hours with fresh medium secreted Aβ1-40 (666.53 ± 29.48 pg/mg protein) and Aβ1-42 (180.19 ± 9.02 pg/mgprotein), as measured through an Aβ ELISA analysis. On the other hand, no exogenous Aβ1-40 and Aβ1-42 wasdetected in N2a cell medium after the cells were cultured for 48 hours. At the concentration of 9 μg·ml-1, 1 CI RJPreduced the Aβ1-40 level from 666.53 ± 29.48 pg/mg to 523.71 ± 31.42 pg/mg protein (P < 0.01, Fig. 4A) and theAβ1-42 level from 180.19 ± 9.02 pg/mg to 120.39 ± 8.60 pg/mg protein (P < 0.001, Fig. 4B), in N2a/App695 cellsmedium. These results suggest that 1 CI RJP affects Aβ metabolism in N2a/APP695 cells.

結果 (繁體中文) 1:

Fraction 1 CIRJP inhibits extracellular Aβ1-40 and Aβ1-42 accumulation in N2a/APP695 cells.
Several RJP fractions were separated from crude RJPs via the HPLC method. We chose the 1 CI fraction as the
active ingredient after preliminary functional verification. Our results showed that 1 CI RJP below 40 μg·ml-1 did
not affect N2a and N2a/APP695 cell viability during 48 hours (Fig. 3). Compared with N2a cells, N2a/APP695
cells produced a large amount of extracellular Aβ1-40 and Aβ1-42. As shown in Fig. 4, N2a/APP695 cells cultured
for 48 hours with fresh medium secreted Aβ1-40 (666.53 ± 29.48 pg/mg protein) and Aβ1-42 (180.19 ± 9.02 pg/mg
protein), as measured through an Aβ ELISA analysis. On the other hand, no exogenous Aβ1-40 and Aβ1-42 was
的N2a細胞培養基後檢測細胞培養48小時。在9微克的濃度·ml-1的，1 CI RJP
降低Aβ1-40水平從666.53±29.48微克/ mg至523.71±31.42 pg / mg蛋白質（P <0.01，圖4A）和
Aβ1-42水平從180.19±9.02皮克/毫克至120.39±8.60 pg / mg蛋白質（P <0.001，圖4B），的N2a / APP695細胞
培養基。這些結果表明，1 CI RJP影響Aβ代謝的N2a / APP695細胞。

結果 (繁體中文) 2:

分數 1 CIRJP 抑制 N2a/APP695 細胞中的細胞外 A+1-40 和 A+1-42 積累。
通過HPLC方法將幾個RJP分數從粗RJP中分離出來。我們選擇了 1 CI 分數作為
經過初步功能驗證後活性成分。我們的結果表明，1 CI RJP 低於40 μgμg=ml-1
不影響N2a和N2a/APP695細胞在48小時內的生存能力（圖3）。與N2a細胞相比，N2a/APP695
細胞產生大量的細胞外A+1-40和A+1-42。如圖 4 所示，N2a/APP695 細胞培養
48小時，使用新鮮介質分泌A+1-40（666.53 ± 29.48 pg/mg蛋白質）和A+1-42 （180.19 = 9.02皮克/毫克）
蛋白質），通過 A+ ELISA 分析進行測量。另一方面，沒有外源性A_1-40和A+1-42
在培養細胞48小時後在N2a細胞培養基中檢測到。濃度為9微克+ml-1，1 CI RJP
將A+1-40水準從666.53 ±29.48皮克/毫克降低至523.71~31.42皮克/毫克蛋白質（P = 0.01，圖4A）和
A+1-42 水準從 180.19 ± 9.02 皮克/毫克到 120.39 = 8.60 pg/mg 蛋白質 （P = 0.001，圖 4B），在 N2a/App695 細胞中
中。這些結果表明，1 CI RJP影響N2a/APP695細胞的A+代謝。

結果 (繁體中文) 3:

第一組分CIRJP抑制N2a/APP695細胞胞外Aβ1-40和Aβ1-42的積累。
採用高效液相色譜法從粗RJP中分離出幾個RJP組分。我們選擇了1個CI分數作為
經初步功能驗證的活性成分。結果表明，在40μg·ml-1以下，1個CI-RJP的劑量為1
在48小時內不影響N2a和N2a/APP695細胞活性（圖3）。與N2a細胞相比，N2a/APP695
細胞產生大量的胞外aβ1-40和aβ1-42。如圖4所示，N2a/APP695細胞培養
新鮮培養48小時，分泌Aβ1-40（666.53±29.48pg/mg蛋白）和Aβ1-42（180.19±9.02pg/mg蛋白）
蛋白質），通過AβELISA分析測定。另一方面，沒有外源性的Aβ1-40和Aβ1-42
培養48h後在N2a細胞培養基中檢測到。濃度為9μg·ml-1，1 CI RJP時
將Aβ1-40水准從666.53±29.48 pg/mg降低到523.71±31.42 pg/mg（P<0.01，圖4A），並且
在N2a/App695細胞中，Aβ1-42水准從180.19±9.02 pg/mg到120.39±8.60 pg/mg蛋白（P<0.001，圖4B）
中等。這些結果提示1 CI-RJP影響N2a/APP695細胞Aβ代謝。