The complete assay was conducted as a two-part plant-plant communication assay in a greenhouse (see above). Four VOCos-receiver or VOCwt-receiver lima bean plants (the first receiver plants) were placed 30 cm apart from four emitter tobacco plants (NtOS2 or WT plants) in the greenhouse for 7 days. The first receiver plant was subsequently treated with 40 T. urticae and used as ‘emitter’ for the second assay started sequentially. During the second assay, four uninfested lima beans (the second receiver plants) were placed 30 cm apart from the four VOCos-receiver or VOCwt-receiver plants in a greenhouse for 1 or 7 days. The second receiver was subsequently exposed to 40 T. urticae for 1 day and subjected to use. Throughout the second assays, transgenic and WT tobacco plants were removed to avoid the effect of their volatiles. Each fumigation was independently replicated 4 times for a given set of experiments.