Cells grown on a sterile cover slip

Cells grown on a sterile cover slip in six-well tissue culture
plates were treated with compounds for a certain range of time. The
culture medium containing compounds was removed, and the cells
were fixed in 4% paraformaldehyde for 10 min. After being washed
twice with PBS, the cells were stained with 0.5 mL of Hoechst
33258 (Beyotime) for 5 min and then againwashed twice with PBS.
The stained nuclei were observed under a Nikon ECLIPSETE2000-S
fluorescence microscope using 350 nm excitation and 460 nm
emission.

結果 (中文) 1:

细胞生长在无菌盖玻片上在六井组织文化板患者一定时间范围的化合物。的培养基中含有化合物已被移除，和单元格被固定在 4%多聚甲醛为 10 分钟。洗涤后两次用 PBS，细胞进行染色 0.5 毫升赫司特公司33258 (Beyotime) 为 5 分钟，然后两次与 PBS againwashed。染色的细胞核观察下尼康 ECLIPSETE2000-S荧光显微镜使用 350 nm 励磁和 460 nm排放量。

結果 (中文) 3:

在六孔组织培养中，在无菌盖层上生长的细胞在一定范围内处理的化合物。化合物的
培养基后，与细胞
被固定在4%多聚甲醛10分钟，用PBS洗两次
后，细胞用0.5 mL Hoechst 33258染色
（碧云天）5 min，然后用PBS
againwashed两次。细胞核的形态观察eclipsete2000-s
尼康荧光显微镜下用350 nm和460 nm
发射。