The actual amount of CA released from each EGC
membrane was measured via HPLC analysis. Based on
the results presented in Figure 1, an optimal CA-extract
concentration of 156 μg/ml was identified for L-929
cell proliferation, and this value was used as the standard.
After the membranes were soaked for 1 day, the
concentrations of asiaticoside released from the EGC
membranes at CA extract concentrations of 31.2 mg/ml
and 46.8 mg/ml were similar to those released from
the membranes with a CA-extract concentration of 156
μg/ml (standard)