the
CA extract at a concentration of 625 μg/ml promoted
fibroblast cell viability and accelerated proliferation.
When L-929 fibroblasts were incubated with 156 μg/ml
of CA for 48 h, the cell proliferation increased up to
70% (Figure 1). However, at the higher concentration
of 2500 μg/ml, the CA extract exerted a cytotoxic and
anti-proliferation effect on the cultured L-929 cells. It
was found that CA extract concentrations in the range of
78–156 μg/ml had the ability to promote cell proliferation.
The toxic intramuscular dose for mice and rabbits is
40–50 mg/kg (Gohil et al., 2010).
Collagen synthesis is important in the wound-healing
process. One of the most important functions of fibroblasts
is the production of collagen (Narayanan et al.,
1989). Fibroblasts were treated separately with the indicated
concentrations of CA extract for 24 h and 72 h.
The amount of collagen synthesized by the fibroblasts
was quantified using the Sircol collag