JC-1 probe was employed to measure

JC-1 probe was employed to measure mitochondrial depolarization
in A549 cells. Briefly, Cells cultured in six-well plates after
indicated treatments were incubated with an equal volume of JC-1
staining solution (5 mg/ml) at 37 C for 20 min and rinsed twice
with PBS. Mitochondrial membrane potentials were monitored by
determining the relative amounts of dual emissions from mitochondrial
JC-1 monomers or aggregates using a Nikon
ECLIPSETE2000-S fluorescent microscope. Mitochondrial depolarization
is indicated by an increase in the green/red fluorescence
intensity ratio.

結果 (中文) 1:

JC-1 号探测器进行测定线粒体去极化A549 细胞。简单地说，在六孔板后的细胞表明的治疗孵育与同等体积的 JC 1染色液 (5 毫克/毫升) 在 37 ℃ 20 分钟，冲洗两次用 PBS。线粒体膜电位进行监测确定双排放线粒体的相对数额JC-1 单体或使用尼康的聚合ECLIPSETE2000 S 荧光显微镜。线粒体去极化绿色，红色荧光增加所示强度比。

結果 (中文) 3:

JC-1探针法检测A549细胞线粒体去极化
。简而言之，培养在六孔板
表明后处理均
JC-1染色液等体积培养细胞（5毫克/毫升）在37  C 20分钟，PBS洗涤2次
。线粒体膜电位的测定，通过测定的相对量的双排放量从线粒体JC-1单体或聚集体使用尼康
eclipsete2000-s荧光显微镜。绿色/红色荧光强度比的增加表示线粒体去极化。