The 15 mL DNA and 3 mL 6 DNA loading buffer mixed samples
were added intowells of 1% agarose gel in TAE containing 0.5 mg/mL
ethidium bromide. Gel electrophoresis was run at 60 V for 2 h. DNA
ladders were finally visualized by a UV light source and documented
by photography. DNA extracted from apoptotic cells
showed a distinct DNA ladder, and DNA from viable cells stayed on
the top of the gel as a high-molecular weight band.