Royal jelly (RJ) is a well-known honeybee product secreted by the hypopharyngeal and mandibular glands of theworker honeybees and has been documented to have a wide-range of usages for promoting human health1,2. RJhas been recognized as having several pharmacological properties, including anti-hypercholesterolemic3, andantioxidant4 abilities among others5,6. The main dry matter of royal jelly consists of royal jelly proteins7. Thedimorphism of honeybee development is known to depend not on genetic differences but on the ingestion ofroyal jelly. A 57-kDa protein in royal jelly has been found to play an important role in inducing the differentiationof honeybee larvae into queens8. Moreover, studies have shown that royal jelly peptides (RJPs) digested from royaljelly proteins have antimicrobial, immunomodulatory, antioxidative, and antihypertensive effects9–12. However,few studies have focused on the neuroprotective effect of RJPs on nerve cells.